Selected publications

Cruz AR, Bentlage AEH, Blonk R, de Haas CJC, Aerts PC, Scheepmaker LM, Bouwmeester IG, Lux A, van Strijp JAG, Nimmerjahn F, van Kessel KPM, Vidarsson G, Rooijakkers SHM. ” oward Understanding How Staphylococcal Protein A Inhibits IgG-Mediated Phagocytosis.” Journal of Immunology 2022 Sep 15;209(6):1146-1155. doi: 10.4049/jimmunol.2200080. (Pubmed: 36002230)

Doorduijn DJ, Lukassen MV, van ’t Wout MFL, Franc V, Ruyken M, Bardoel BW, Heck AJR, . “Soluble MAC is primarily released from MAC-resistant bacteria that potently convert complement component C5.” Elife 2022 Aug 10;11:e77503. doi: 10.7554/eLife.77503. (Pubmed: 35947526)

de Vor L, Beudeker CR, Flier A, Scheepmaker LM, Aerts PC, Vijlbrief DC, Bekker MN, Beurskens FJ, van Kessel KPM, de Haas CJC, Rooijakkers SHM, van der Flier M. ” Monoclonal antibodies effectively potentiate complement activation and phagocytosis of Staphylococcus epidermidis in neonatal human plasma.” Front Immunology 2022 Jul 29;13:933251. doi: 10.3389/fimmu.2022.933251. (Pubmed: 35967335)

de Vor L, van Dijk B, van Kessel K, Kavanaugh JS, de Haas C, Aerts PC, Viveen MC, Boel EC, Fluit AC, Kwiecinski JM, Krijger GC, Ramakers RM, Beekman FJ, Dadachova E, Lam MG, Vogely HC, van der Wal BC, van Strijp JA, Horswill AR, Weinans H, Rooijakkers SH. “Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro and in vivo biofilm.” Elife 2022 Jan 6;11:e67301. doi: 10.7554/eLife.67301. (Pubmed: 34989676)

Doorduijn DJ, Heesterbeek DAC, Ruyken M, de Haas CJC, Stapels DAC, Aerts PC, Rooijakkers SHM, Bardoel BW. “Polymerization of C9 enhances bacterial cell envelope damage and killing by membrane attack complex pores.” PLoS Pathog. 2021 Nov 9;17(11):e1010051. doi: 10.1371/journal.ppat.1010051. eCollection 2021 Nov. (Pubmed: 34752492)

Zwarthoff SA, Widmer K, Kuipers A, Strasser J, Ruyken M, Aerts PC, de Haas CJC, Ugurlar D, den Boer MA, Vidarsson G, van Strijp JAG, Gros P, Parren PWHI, van Kessel KPM, Preiner J, Beurskens FJ, Schuurman J, Ricklin D, Rooijakkers SHM. ” C1q binding to surface-bound IgG is stabilized by C1r2s2 proteases.” Proc Natl Acad Sci USA. 2021 Jun 29;118(26):e2102787118. doi: 10.1073/pnas.2102787118. (Pubmed: 34155115)

Zwarthoff SA, Magnoni S, Aerts PC, van Kessel KPM, Rooijakkers SHM. “Method for Depletion of IgG and IgM from Human Serum as Naive Complement Source.” Methods Mol Biol. 2021;2227:21-32. doi: 10.1007/978-1-0716-1016-9_2. (Pubmed: 33847927)

Cruz AR, Boer MAD, Strasser J, Zwarthoff SA, Beurskens FJ, de Haas CJC, Aerts PC, Wang G, de Jong RN, Bagnoli F, van Strijp JAG, van Kessel KPM, Schuurman J, Preiner J, Heck AJR, Rooijakkers SHM. “Staphylococcal protein A inhibits complement activation by interfering with IgG hexamer formation.” Proc Natl Acad Sci USA. 2021 Feb 16;118(7):e2016772118. doi: 10.1073/pnas.2016772118. (Pubmed: 33563762)

Boero E, Brinkman I, Juliet T, van Yperen E, van Strijp JAG, Rooijakkers SHM, van Kessel KPM. “Use of Flow Cytometry to Evaluate Phagocytosis of Staphylococcus aureus by Human Neutrophils.” Front Immunol. 2021 Feb 19;12:635825. doi: 10.3389/fimmu.2021.635825. (Pubmed: 33679791)

Heesterbeek DAC, Muts RM, van Hensbergen VP, de Saint Aulaire P, Wennekes T, Bardoel BW, van Sorge NM, Rooijakkers SHM. “Outer membrane permeabilization by the membrane attack complex sensitizes Gram-negative bacteria to antimicrobial proteins in serum and phagocytes.”
PLoS Pathog. 2021 Jan 22;17(1):e1009227. doi: 10.1371/journal.ppat.1009227.  (Pubmed: 33481964)

Doorduijn DJ, Bardoel BW, Heesterbeek DAC, Ruyken M, Benn G, Parsons ES, Hoogenboom BW, Rooijakkers SHM. “Bacterial Killing by Complement Requires Direct Anchoring of Membrane Attack Complex Precursor C5b-7.” PLoS Pathog. 2020 Jun 22;16(6):e1008606. doi: 10.1371/journal.ppat.1008606. (Pubmed: 32569291)

de Vor L, Rooijakkers SHM, van Strijp JAG. “Staphylococci evade the innate immune response by disarming neutrophils and forming biofilms.” FEBS Letters. 2020 Mar 6. doi: 10.1002/1873-3468.13767. (Pubmed: 32144756)

Doorduijn DJ, Rooijakkers SHM, Heesterbeek DAC. “How the Membrane Attack Complex Damages the Bacterial Cell Envelope and Kills Gram-Negative Bacteria.” Bioessays. 2019 Oct;41(10):e1900074. (Pubmed: 31452228)

Heesterbeek DAC, Martin NI, Velthuizen A, Duijst M, Ruyken M, Wubbolts R, Rooijakkers SHM, Bardoel BW. “Complement-dependent outer membrane perturbation sensitizes Gram-negative bacteria to Gram-positive specific antibiotics.” Scientific Reports. 2019 Feb 28;9(1):3074. doi: 10.1038/s41598-019-38577-9. (Pubmed: 30816122)

Heesterbeek DA, Bardoel BW, Parsons ES, Bennett I, Ruyken M, Doorduijn DJ, Gorham RD Jr, Berends ET, Pyne AL, Hoogenboom BW, Rooijakkers SH. “Bacterial killing by complement requires membrane attack complex formation via surface-bound C5 convertases.” The EMBO Journal. 2019 Jan 14. pii: e99852. doi: 10.15252/embj.201899852. (Pubmed: 30643019)

Heesterbeek DAC, Angelier ML, Harrison RA, Rooijakkers SHM. “Complement and bacterial infections; from molecular mechanisms to therapeutic applications”. Journal of Innate Immunity. 2018;10(5-6):455-464. doi: 10.1159/000491439. (Pubmed: 30149378)

Zwarthoff SA, Berends ETM, Mol S, Ruyken M, Aerts PC, Józsi M, de Haas CJC, Rooijakkers SHM, Gorham RD Jr. “Functional Characterization of Alternative and Classical Pathway C3/C5 Convertase Activity and Inhibition Using Purified Models.” Frontiers in Immunology. 2018 Jul 23;9:1691. doi: 10.3389/fimmu.2018.01691. (Pubmed: 30083158)

Paganelli FL, Leavis HL, He S, van Sorge NM, Payré C, Lambeau G, Willems RJL, Rooijakkers SHM. “Group IIA secreted phospholipase A2 in human serum kills commensal but not clinical Enterococcus faecium isolates.” Infection and Immunity. 2018 Jul 23;86(8). pii: e00180-18. doi: 10.1128/IAI.00180-18. (Pubmed: 29784864)

de Jong NWM, Vrieling M, Garcia BL, Koop G, Brettmann M, Aerts PC, Ruyken M, van Strijp JAG, Holmes M, Harrison EM, Geisbrecht BV, Rooijakkers SHM. “Identification of a staphylococcal complement inhibitor with broad host specificity in equid Staphylococcus aureus strains.” Journal of Biological Chemistry 2018 Mar 23;293(12):4468-4477. doi: 10.1074/jbc.RA117.000599 (Pubmed: 29414776)

Bennett KM, Rooijakkers SH, Gorham RD Jr. “Let’s Tie the Knot: Marriage of Complement and Adaptive Immunity in Pathogen Evasion, for Better or Worse.” Frontiers in Microbiology 2017 Jan 31;8:89. doi: 10.3389/fmicb.2017.00089. (Pubmed: 28197139)

Garcia BL, Zwarthoff SA, Rooijakkers SH, Geisbrecht BV. “Novel Evasion Mechanisms of the Classical Complement Pathway.” Journal of Immunology 2016 Sep 15;197(6):2051-60: doi: 10.4049/jimmunol.1600863 (Pubmed: 27591336)

Kuipers A, Stapels DA, Weerwind LT, Ko YP, Ruyken M, Lee JC, van Kessel KP, Rooijakkers SH. “The S. aureus polysaccharide capsule and Efb-dependent fibrinogen shield act in concert to protect against phagocytosis.”  Microbiology 2016 Apr 25. doi: 10.1099/mic.0.000293 (Pubmed 27112346)

Stapels DA, Kuipers A, von Köckritz-Blickwede M, Ruyken M, Tromp AT, Horsburgh MJ, de Haas CJ, van Strijp JA, van Kessel KP, Rooijakkers SH. “Staphylococcus aureus protects its immune-evasion proteins against degradation by neutrophil serine proteases.” Cellular Microbiology 2016 Apr;18(4):536-45, doi: 10.1111/cmi.12528 (Pubmed: 26418545)

Berends ET, Gorham RD Jr, Ruyken M, Soppe JA, Orhan H, Aerts PC, de Haas CJ, Gros P, Rooijakkers SH. “Molecular insights into the surface-specific arrangement of complement C5 convertase enzymes”. BMC Biology 2015 Nov 9;13:93 (Pubmed: 26552476)

Berends ET, Mohan S, Miellet WR, Ruyken M, Rooijakkers SH. “Contribution of the complement Membrane Attack Complex to the bactericidal activity of human serum.” Molecular Immunology 2015 Jun;65(2):328-35. doi: 10.1016/j.molimm.2015.01.020. (Pubmed: 25725315)

Stapels DA, Geisbrecht BV, Rooijakkers SH. “Neutrophil serine proteases in antibacterial defense”. Current Opinion in Microbiology. 2014 Nov 18;23:42-48. (Pubmed: 25461571)

Woehl JL, Stapels DA, Garcia BL, Ramyar KX, Keightley A, Ruyken M, Syriga M, Sfyroera G, Weber AB, Zolkiewski M, Ricklin D, Lambris JD, Rooijakkers SH, Geisbrecht BV. “The Extracellular Adherence Protein from the C3 Proconvertase.”Journal of Immunology. 2014 Dec 15;193(12):6161-71. (Pubmed: 25381436)

Stapels DA, Ramyar KX, Bischoff M, von Köckritz-Blickwede M, Milder FJ, Ruyken M, Eisenbeis J, McWhorter WJ, Herrmann M, van Kessel KP, Geisbrecht BV, Rooijakkers SH. “Staphylococcus aureus secretes a unique class of neutrophil serine protease inhibitors”.Proc Natl Acad Sci USA. 2014 Sep 9;111(36):13187-92. (Pubmed: 25161283)

Berends ET, Kuipers A, Ravesloot M, Urbanus RT and Rooijakkers SH. ”Bacteria under stress by complement ant coagulation” FEMS Microbiology Reviews. 2014;38(6):1146-71. (Pubmed: 2506546)

van Strijp JA, Rooijakkers SH. Entrapment exploited. Trends Microbiology. 2014;22(2):55-7. (Pubmed: 24387831)

Ko YP, Kuipers A, Freitag CM, Jongerius I, Medina E, van Rooijen WJ, Spaan AN, van Kessel KP, Höök M, Rooijakkers SH. Phagocytosis escape by a Staphylococcus aureus protein that connects complement and coagulation proteins at the bacterial surface. PLoS Pathogens. 2013 Dec;9(12):e1003816. (Pubmed: 24348255)

Berends ET, Dekkers JF, Nijland R, Kuipers A, Soppe JA, van Strijp JA, Rooijakkers SH. Distinct localization of the complement C5b-9 complex on Gram-positive bacteria. Cell Microbiol. 2013;15(12):1955-68. (Pubmed: 23869880)

Rooijakkers SH#, Geiss-Liebisch S# , Beczala A, Sanchez-Carballo P, Kruszynska K, Repp C, Sakinc T, Vinogradov E, Holst O, Huebner J, Theilacker C. “Secondary Cell Wall Polymers of Enterococcus faecalis Are Critical for Resistance to Complement Activation via Mannose-binding Lectin”.Journal of Biological Chemistry. 2012 Nov 2;287(45):37769-77. #Equal contribution (Pubmed: 2290821)

Laarman AJ, Mijnheer G, Mootz JM, van Rooijen WJ, Ruyken M, Malone CL, Heezius EC, Ward R, Milligan G, van Strijp JA, de Haas CJ, Horswill AR, van Kessel KP, Rooijakkers SH. “Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis”.EMBO Journal. 2012 Aug 29;31(17):3607-19. (Pubmed: 22850671)

Jongerius I, von Köckritz-Blickwede M, Horsburgh MJ, Ruyken M, Nizet V, Rooijakkers SH.”Staphylococcus aureus virulence is enhanced by secreted factors that block innate immune defenses”.Journal of Innate Immunity. 2012;4(3):301-11. (Pubmed: 22327617)

Laarman AJ, Bardoel BW, Ruyken M, Fernie J, Milder FJ, van Strijp JA, Rooijakkers SH. “Pseudomonas aeruginosa alkaline protease blocks complement activation via the classical and lectin pathways”.Journal of Immunology. 2012 Jan 1;188(1):386-93. (Pubmed: 22131330)

Laarman AJ, Ruyken M, Malone CL, van Strijp JA, Horswill AR, Rooijakkers SH.“Staphylococcus aureus metalloprotease aureolysin cleaves complement C3 to mediate immune evasion.” Journal of Immunology. 2011 Jun 1;186(11):6445-53. (Pubmed: 21502375)

Rooijakkers SH, Rasmussen SL, McGillivray SM, Bartnikas TB, Mason AB, Friedlander AM, Nizet V. “Human transferrin confers serum resistance against Bacillus anthracis.”Journal of Biological Chemistry. 2010 Sep 3;285(36):27609-13. (Pubmed: 20615872)

Jongerius I, Garcia BL, Geisbrecht BV, van Strijp JA, Rooijakkers, SH. “Convertase inhibitory properties of the staphylococcal extracellular complement binding protein.” Journal of Biological Chemistry. 2010 Mar 19. (Pubmed: 20304920)

Jongerius I, Puister M, Wu J, Ruyken M, van Strijp JA and Rooijakkers SH. “Staphylococcal complement inhibitor modulates phagocyte responses by dimerization of convertases.” Journal of Immunology. 2010 Jan 184: 420-425 (Pubmed: 19949103)

Rooijakkers SH, Wu J, Ruyken M, van Domselaar R, Planken KL, Tzekou A, Ricklin D, Lambris JD, Janssen BJ, van Strijp JA, Gros P. “Structural and functional implications of the alternative complement pathway C3 convertase stabilized by a staphylococcal inhibitor.” Nature Immunology. 2009 Jul;10(7):721-7. (Pubmed: 19503103)

Jongerius I, Köhl J, Pandey MK, Ruyken M, Van Kessel KPM, Van Strijp JAG and Rooijakkers SH. “Staphylococcal complement evasion by various convertase-blocking molecules”.Journal of Experimental Medicine, 2007 Oct 1;204(10):2461-71. (Pubmed: 17893203)

Rooijakkers SH, Van Kessel KPM, Van Strijp JAG. “Staphylococcal Innate Immune evasion”. Trends in Microbiology, 2005, Dec 13(12): 596-601. (Pubmed: 16242332)

Rooijakkers SH, Ruyken M, Roos A, Daha MR, Presanis J, Sim R, Van Wamel WJB, Van Kessel KP, Van Strijp JAG. “Immune evasion by a staphylococcal complement inhibitor that acts on C3 convertases”. Nature Immunology, 2005, Sep 6(9): 920-927. (Pubmed: 16086019)